Reporter

Part:BBa_K5319674

Designed by: YuFei Xie   Group: iGEM24_ZJU-China   (2024-09-30)


pJUMP42x-2a-TRS/RS-sfGFP

It's a readout plasmid adapting to HYER based on the vector jump42x-2a-sfGFP, with a pair of recognition sites flanking loxp sequence upstream of sfGFP. The recognition sequence is composed of 6-nt target recognition site(TRS) and 14-nt recruiting sequence(RS), referred to as TRS/RS. Ideally, the RS-inserted ribozyme HYER can specifically locate itself to TRS/RS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 10
    Illegal NheI site found at 33
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 101
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 147


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Parameters
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